Unraveling the Complexity of Ivonescimab Binding

Video

Published: December 1, 2025

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Advances in antibody therapeutics have driven the development of complex bi- and multi-specific modalities, creating opportunities for next-generation treatments. However, their intricate binding behaviors pose significant challenges for conventional analytical methods, making it difficult to determine stoichiometry and binding affinity accurately.

Dr. Nina Jajcanin Jozic, Senior Applications Scientist at Refeyn, joins this Teach Me in 10 to demonstrate how single-molecule mass photometry overcomes these analytical challenges. She describes how it delivers fast, quantitative insights into the stoichiometry and multi-target interactions of multi-specific antibodies, presenting mass photometry analysis of the bispecific antibody ivonescimab as an example.

Watch this episode to discover:

How new modalities offer new therapeutic opportunities while bringing analytical challenges
How mass photometry works and its advantages as a bioanalytic tool for new antibody modalities
How it reveals real-time binding interactions, even for complex, next-generation antibodies with more than one antigen binding site

Further Resources:

Mass Photometry Handbook
Application note: Rapid Analysis of Bispecific Antibody Stability and Target Binding by Mass Photometry
Application note: Comparing Mass Photometry and SEC for Antibody Aggregation Assessment
On-demand webinar: Explore the Binding Mechanism of Bispecific Antibody Ivonescimab With Mass Photometry
Refeyn's latest pre-print: Mass Photometry Reveals Stoichiometry and Binding Dynamics of Bispecific Tetravalent Anti-VEGF-PD-1 Antibody Ivonescimab

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Meet the Author

Laura Hemmingham, PhD

Scientific Content Producer

Laura holds a BSc in Zoology from Queen Mary University of London, an MSc in Palaeoanthropology from UCL, and a PhD in Environmental Sciences from Royal Holloway, University of London. Her academic journey has been fueled by a broad interest in the life sciences and a passion for making complex science accessible to diverse audiences. Before joining Technology Networks in 2025, she gained experience across several areas of science communication, including e-learning, external communications, and science writing. Her work has focused on translating technical research into engaging, understandable content tailored for both specialist and general audiences. As a Scientific Content Producer for custom video content at Technology Networks, Laura is particularly interested in bridging the gap between scientific discovery and public engagement through creative storytelling. Her work aims to support scientific literacy while highlighting the real-world impact of research and innovation.

Interviewing

Nina Jajcanin Jozic, PhD

Senior Applications Scientist

Nina joined Refeyn in 2024, where she currently works on mass photometry applications in new modalities, primarily with bispecific antibodies. Before that, Nina worked at Oxford Nanoimaging developing innovative workflows and tools for super resolution microscopy. Nina has extensive cell biology experience from her postdoctoral research work at University of Oxford, PhD in Medicinal Chemistry and Biochemistry and MSc in Biology and Chemistry.