The Simple Path To Proteomics in Just Nine Steps

Proteomics for all No new instruments needed - works with a wide range of mid and highthroughput sequencers. Broad proteome coverage and deep profiling of inflammation – with around 1000 proteins highly representative of known disease and proteogenomic associations. Comprehensive data analysis and quality control software for all levels of expertise. Affordable library prep that seamlessly integrates with NGS workflows. Don’t get lost on the path to proteomics DAY 1 DAY 2 STEP 1 STEP 8 STEP 2 STEP 9 STEP 4 STEP 5 STEP 6 STEP 7 STEP 3 Using existing NGS infrastructure and expertise, labs can run a proteomics workflow in a short timeframe with minimal hands-on time, even without prior proteomics experience. The complete library preparation protocol can be completed in 1.5 days, with typically less than 2.5 hours of hands-on time. Dilute samples and controls. (86 samples + 10 controls) Assess quality of the library through an electrophoresis platform and send library for NGS sequencing. Incubate overnight at room temperature. Hands on time ~1hr ~2hr Add diluted samples to a pre-loaded incubation plate containing all pre-mixed and dried-down reagents needed for the reaction, including antibodies. QC data with dedicated software and analyze results with Olink® Insight, an open-access tool for study design and data analysis Add PCR mastermix to incubated samples. Run PCR. Pool amplified samples into one tube. Use magnetic beads for the library purification. How does it work? All Olink panels are powered by the highly trusted Proximity Extension Assay (PEA), a unique technology that combines antibody-based assays with PCR. This approach enables multiplex protein biomarker analysis with exceptional specificity, high sensitivity, and minimal sample volume requirements Two matched antibodies labelled with unique oligonucleotides simultaneously bind to a target protein in solution. In close proximity and upon specific antibody binding, the oligonucleotides hybridize and are extended by DNA polymerase. The newly created double-stranded DNA barcode is amplified by PCR, ready for readout by NGS. $ 5 ( " " " " " " ( ( 5 5 5 5 5 $ $ " " " " " " ( ( ( 5 5 5 5 5 5 $ $ $ Find your way to proteomics: click here to learn more Easy protocol with pre-mixed reagents. Add proteomics to your next-generation sequencing workflow in just nine steps. Proteomics can complement genomics results by giving dynamic, real-time insights into biological functions and disease mechanisms. Integrating genomics with proteomics enables broad applications across areas such as precision medicine, biomarker research and drug discovery. Proteomics has long been seen as complex, costly, and confined to specialized labs. But what if you could generate high-quality proteomics data using existing standard lab molecular biology equipment and NGS infrastructure? This infographic explores a workflow that can bring proteomics to any lab in nine easy steps, with Olink Reveal. The Simple Path to Proteomics with Olink® Reveal QC AND NGS Created with BioRender.com Created with BioRender.com Created with BioRender.com Created with BioRender.com Created with BioRender.com Created with BioRender.com Created with BioRender.com Created with BioRender.com Created with BioRender.com Created with BioRender.com Hands on t ime ~30 min Hands on time ~1hr © 2025 Olink Proteomics AB, part of Thermo Fisher Scientific. Olink products and services are For Research Use Only. Not for use in diagnostic procedures. All information in this document is subject to change without notice. This document is not intended to convey any warranties, representations and/or recommendations of any kind, unless such warranties, representations and/or recommendations are explicitly stated. Olink assumes no liability arising from a prospective reader’s actions based on this document. OLINK, NPX, PEA, INSIGHT and the Olink logotype are trademarks registered, or pending registration, by Olink Proteomics AB. All third-party trademarks are the property of their respective owners. Olink products and assay methods are covered by several patents and patent applications https://www.olink.com/legal/patents.