Understanding the complexity of cancer biology requires insights that go beyond DNA or RNA alone. Traditional approaches often silo genotype and transcriptomic data, obscuring the true drivers of tumor evolution and therapeutic resistance.
Integrating DNA and RNA analysis at single-cell resolution opens new doors for discovery.
This whitepaper explores how targeted single-cell assays can unify genotypic and transcriptomic data to expose clonal structures, track rare populations and reveal resistance mechanisms with unprecedented clarity.
Download this whitepaper to explore:
- How integrated genotype and gene expression profiling enhances cancer research
- The role of single-cell analysis in uncovering therapeutic resistance and tumor evolution
- Options for tailoring panels to your specific research targets
A New Paradigm in Precision is Coming With Custom Assays for Targeted Genotype & Gene ExpressionComing Soon: Single-cell targeted genotype and gene expression—all in one assay Understanding cancer requires more than DNA mutations or RNA expression alone. Tumor evolution, clonal dynamics, and mechanisms of resistance often remain hidden when data is siloed. What if you could directly link genotype to gene expression — in the same cell? Our upcoming single-cell targeted DNA + RNA assay is designed to give you unprecedented resolution into cancer heterogeneity. Advance your research by efficiently identifying: MISSIONBIO.COMTranslate insight into action with fully interpreted results This experiment highlights the power of Tapestri and its ability to distinguish cell types in cell lines and PBMCs. T47D (breast cancer) and A549 (lung cancer) were chosen to highlight the variable genotypic profiles of each disease, while PBMCs were included to show how granularly the cell types within the sample type could be distinguished and categorized. Figure 1: The ‘Genotype’ subplot displays cells from the multiplexed run, colored according to their genotype-level information derived from genotype demultiplexing, which identifies samples based on genotype variants. The ‘RNA Cell Type’ subplot shows the same cells colored by clustering results obtained from gene expression profiles of the targeted gene panel or probes. Panel options tailored to your research: Genotype: Choose from Mission Bio’s extensive catalog of pre-designed, validated panels—or create a custom panel with up to 350 amplicons to match your specific targets. Gene Expression Targets: Use the Tapestri Targeted Single-cell Gene Expression Core Panel (covering 234 genes), customize a panel of up to 50 gene targets for focused expression profiling, or use a combination of both. Your data will be analyzed by experienced bioinformaticians and delivered in a comprehensive report covering: — Single-nucleotide variants (SNVs), indels, CNVs, and translocations — Gene expression profiles, co-occurrence and zygosity of mutations within individual cells — Identification of rare cell populations and clonal structures — Integration of genotype and gene expression data to reveal therapeutic resistance mechanisms and tumor evolution MISSIONBIO.COM
Tapestri Single-cell Targeted Gene Expression panel
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