Gentle and Rapid Detachment of Even Delicate Adherent Cells

Detachin™ Cell Detachment Solution from Amsbio provides a superior alternative to Trypsin/EDTA for gentle detachment of adherent cells from in vitro growth vessels.

Widely cited in recent published papers^{1, 2, 3, 4} - Detachin™ is proven to quickly deliver gentle detachment of a wide variety of adherent cells, including primary cell types and delicate culture systems, from any tissue culture plasticware. These papers involving primary immune cells, patient-derived cancer models, engineered mammalian lines and high-throughput screening systems, provide further evidence for gentle cell detachment using Detachin - which is shown to maintain phenotypic accuracy and downstream assay reproducibility. In addition, this growing body of evidence demonstrates how Detachin™ enhances cell integrity in diverse and demanding next-generation adherent cell workflows.

The proprietary Detachin™ formulation contains protease and collagenase activities in an isotonic phosphate buffer solution with EDTA. It has been tested successfully on many different primary cells and cell lines, including: bone marrow cells, stem cells, fibroblasts, hepatocytes, mouse germ cells, keratinocytes, macrophages, chick embryo cells, neuronal cells, vascular endothelial cells, A-375, BHK, CHO, COS, D54, HEK293, HeLa, L-929, M-24, MG-63, MRC-5, NIH-3T3, NT-2 cells, U-251, Vero, Sf9 insect cells, and many others.

Stable at 4°C for 2 months, not only does Detachin™ Cell Detachment Solution offer unmatched cell viability, but it also produces no mammalian or bacterial byproducts meaning there is no need to wash your detached cells. Offered in a range of convenient formats and sizes - Detachin™ provides an economic solution to labs regularly tasked with cell detachment protocols.